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Journal / Immunology and Infection / 流式细胞术分析人髓系U937细胞中SAMHD1限制性…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
流式细胞术分析人髓系U937细胞中SAMHD1限制性
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Journal (Immunology and Infection)
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
Journal (Immunology and Infection)
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
 
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流式细胞术分析人髓系U937细胞中SAMHD1限制性

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

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这里描述的是一种确定HIV-1限制程度的既定方法，由细胞抑制蛋白SAMHD1。人髓系U937细胞用共表达YFP的SAMHD1表达载体转导，分化，然后用HIV-RFP攻击。限制水平由流式细胞术分析确定。

Tags

免疫学和感染，第172期，人类免疫缺陷病毒-1型（HIV-1），SAMHD1，限制性，骨髓细胞，流式细胞术，逆转录病毒
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