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Journal / Biochemistry / Herstellung rekombinanter PRMT-Proteine unter…
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Biochemistry
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JoVE Journal Biochemistry
Herstellung rekombinanter PRMT-Proteine unter Verwendung des Baculovirus Expression Vector System
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Herstellung rekombinanter PRMT-Proteine unter Verwendung des Baculovirus Expression Vector System

Article DOI: 10.3791/62510-v • 08:57 min • July 17th, 2021
July 17th, 2021 •
1, 1
1Structural Genomics Consortium, University of Toronto

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Das Baculovirus Expression Vector System (BEVS) ist eine robuste Plattform für das Expressionsscreening und die Produktion von Protein-Arginin-Methyltransferasen (PRMTs), die für biochemische, biophysikalische und strukturelle Studien verwendet werden sollen. Für die meisten PRMTs und andere Proteine von Interesse, die eine eukaryotische Expressionsplattform benötigen, können Milligrammmengen an Material produziert werden.

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Biochemistry Protein Arginin methyltransferasen PRMTs Baculovirus Expression Vector System BEVS Spodoptera frugiperda Insektenzellen rekombinantes Protein Transfektionsreagenzien Suspensionskultur von Baculovirus-infizierten Insektenzellen SCBIIC 2,8 L Fernbach Kulturkolben 2,5 L Tunairkolben 5 L Reagenzflaschen
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