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Journal / Biochemistry / Perfil proteómico de EPS-Urine a través de la…
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Biochemistry
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JoVE Journal Biochemistry
Perfil proteómico de EPS-Urine a través de la Digestión FASP y análisis independiente de datos
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Perfil proteómico de EPS-Urine a través de la Digestión FASP y análisis independiente de datos

Article DOI: 10.3791/62512-v • 14:48 min • May 8th, 2021
May 8th, 2021 •
1, 1, 2, 3, 3, 1, 4, 1
1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 2Department of Health Science, Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro

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Aquí, presentamos un protocolo de digestión en filtro optimizado con información detallada sobre lo siguiente: digestión de proteínas, purificación de péptidos y análisis de adquisición independiente de datos. Esta estrategia se aplica al análisis de muestras de secreciones prostáticas-orina expresadas y permite una alta cobertura de proteoma y un perfil sin etiquetas de bajo valor del proteoma urinario.

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Bioquímica Número 171
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