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Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
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Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Article DOI: 10.3791/62552-v • 09:09 min • April 27th, 2021
April 27th, 2021 •
1, 2, 1,3
1Department of Biology, Virginia Commonwealth University, 2VCU Lipidomics/Metabolomics Shared Resource, Virginia Commonwealth University School of Medicine, 3Massey Cancer Center

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Summary
Automatic Translation

Sphingolipids are bioactive metabolites with well-established roles in human disease. Characterizing alterations in tissues with mass-spectrometry can reveal roles in disease etiology or identify therapeutic targets. However, the OCT-compound used for cryopreservation in biorepositories interferes with mass-spectrometry. We outline methods to analyze sphingolipids in human tissues embedded in OCT with LC-ESI-MS/MS.

Tags

Human Tissues Optimal Cutting Temperature Compound (OCT) Mass Spectrometry Analysis Cryo-preservation Agent Tandem Liquid Chromatography Mass Spectrometry Sphingolipids Tissue Drying Wicks Tissue Washing Tissue Weighing Surgical Scissors Methanol Laboratory Grade Tissue Powder-free Gloves Pipette Tip Ultrapure Deionized Water Human Lung Tissue Incubate Vortex
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