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Fremstilling av menneskelig vev innebygd i optimal skjæretemperaturforbindelse for massespektrometrianalyse
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Fremstilling av menneskelig vev innebygd i optimal skjæretemperaturforbindelse for massespektrometrianalyse

Article DOI: 10.3791/62552-v • 09:09 min • April 27th, 2021
April 27th, 2021 •
1, 2, 1,3
1Department of Biology, Virginia Commonwealth University, 2VCU Lipidomics/Metabolomics Shared Resource, Virginia Commonwealth University School of Medicine, 3Massey Cancer Center

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Sfingolipider er bioaktive metabolitter med veletablerte roller i human sykdom. Karakterisering av endringer i vev med massespektrometri kan avsløre roller i sykdomsetiologi eller identifisere terapeutiske mål. Imidlertid forstyrrer OCT-forbindelsen som brukes til kryopreservering i biorepositorier massespektrometri. Vi skisserer metoder for å analysere sfingolipider i humant vev innebygd i OCT med LC-ESI-MS / MS.

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Kreftforskning utgave 170 lipidomikk sfingolipid biorepositorium OCT lungekreft lungeadenokarsinom ikke-småcellet lungekreft svulst optimal skjæretemperaturforbindelse LC-ESI-MS / MS massespektrometri
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