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Bewertung der globalen DNA-Doppelstrang-Endsektion mittels BrdU-DNA-Markierung in Verbindung mit Cell Cycle Discrimination Imaging
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Bewertung der globalen DNA-Doppelstrang-Endsektion mittels BrdU-DNA-Markierung in Verbindung mit Cell Cycle Discrimination Imaging

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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Im vorliegenden Protokoll demonstrieren wir, wie die DNA-Doppelstrang-Endsektion während der S/G2-Phase des Zellzyklus mit einer immunfluoreszenzbasierten Methode visualisiert werden kann.

Tags

Biologie Ausgabe 170 BrdU-Herde Immunfluoreszenz DNA-Endresektion homologe Rekombination PARP-1 PARP-Inhibitoren PARP-Inhibitorresistenz BMN673
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