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JoVE Journal Chemistry
Identifikation af antibakterielle immunitetsproteiner i Escherichia coli ved hjælp af MALDI-TOF-TOF-MS/MS og Top-Down Proteomisk analyse
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Identifikation af antibakterielle immunitetsproteiner i Escherichia coli ved hjælp af MALDI-TOF-TOF-MS/MS og Top-Down Proteomisk analyse

Article DOI: 10.3791/62577-v • 09:26 min • May 23rd, 2021
May 23rd, 2021 •
1, 1
1Produce Safety & Microbiology, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture

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Her præsenterer vi en protokol for hurtig identifikation af proteiner produceret af genomisk sekventeret patogene bakterier ved hjælp af MALDI-TOF-TOF tandem massespektrometri og top-down proteomisk analyse med software udviklet in-house. Metastable proteinioner fragment på grund af aspartsyre effekt og denne specificitet udnyttes til protein identifikation.

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