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Journal / Chemistry / 使用MALDI-TOF-TOF-MS/MS和自上而下的蛋白质组学分析鉴定 大肠杆菌…
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使用MALDI-TOF-TOF-MS/MS和自上而下的蛋白质组学分析鉴定 大肠杆菌 中的抗菌免疫蛋白
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使用MALDI-TOF-TOF-MS/MS和自上而下的蛋白质组学分析鉴定 大肠杆菌 中的抗菌免疫蛋白

Article DOI: 10.3791/62577-v • 09:26 min • May 23rd, 2021
May 23rd, 2021 •
1, 1
1Produce Safety & Microbiology, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture

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在这里，我们提出了一种方案，用于使用MALDI-TOF-TOF串联质谱和自上而下的蛋白质组学分析以及内部开发的软件快速鉴定基因组测序的致病细菌产生的蛋白质。亚稳态蛋白离子片段由于天冬氨酸效应和这种特异性被用于蛋白质鉴定。

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《化学》，第171期，
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