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JoVE Journal Chemistry
Identifizierung antibakterieller Immunitätsproteine in Escherichia coli mittels MALDI-TOF-TOF-MS/MS und Top-Down-Proteomanalyse
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Journal (Chemistry)
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
Journal (Chemistry)
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
 
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Identifizierung antibakterieller Immunitätsproteine in Escherichia coli mittels MALDI-TOF-TOF-MS/MS und Top-Down-Proteomanalyse

Article DOI: 10.3791/62577-v • 09:26 min • May 23rd, 2021
May 23rd, 2021 •
1, 1
1Produce Safety & Microbiology, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture

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Hier stellen wir ein Protokoll zur schnellen Identifizierung von Proteinen vor, die von genomisch sequenzierten pathogenen Bakterien mittels MALDI-TOF-TOF-Tandem-Massenspektrometrie und Top-Down-Proteomanalyse mit eigens entwickelter Software produziert werden. Metastabile Proteinionen fragmentieren aufgrund der Asparaginsäurewirkung und diese Spezifität wird für die Proteinidentifizierung ausgenutzt.

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Chemie Heft 171
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