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Journal / Genetics / Mikroinjektionsmethode für Anopheles gambiae…
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JoVE Journal Genetics
Mikroinjektionsmethode für Anopheles gambiae Embryonen
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Mikroinjektionsmethode für Anopheles gambiae Embryonen

Article DOI: 10.3791/62591-v • 05:39 min • July 7th, 2021
July 7th, 2021 •
1, 1, 2, 1,2
1Department of Microbiology & Molecular Genetics, University of California, Irvine, 2Department of Molecular Biology & Biochemistry, University of California, Irvine

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Mikroinjektionstechniken sind unerlässlich, um exogene Gene in die Genome von Moskitos einzuführen. Dieses Protokoll erklärt eine Methode, die vom James-Labor verwendet wird, um DNA-Konstrukte in Anopheles gambiae-Embryonen zu mikroinjizieren, um transformierte Moskitos zu erzeugen.

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Genetik Ausgabe 173 Transgenesevektoren Genexpression Promotoranalyse Mücke Mikroinjektion Gentechnik
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