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Journal / Genetics / Preparação amostral para análise bioinformática…
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Genetics
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JoVE Journal Genetics
Preparação amostral para análise bioinformática da metilação de DNA
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Preparação amostral para análise bioinformática da metilação de DNA: Estratégia de Associação para Obesidade e Estudos de Traços Relacionados

Article DOI: 10.3791/62598-v • 14:56 min • May 6th, 2022
May 6th, 2022 •
*1, *1, 1, 2,3, 1, 4, 5, 1, 1, 6, 7, 8, 9, 6
1Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, 2Centre for Computational Biology, University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport, University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, 7Faculty of Pharmaceutical Sciences, University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo
* These authors contributed equally

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O presente estudo descreve o fluxo de trabalho para gerenciar dados de metilação de DNA obtidos por tecnologias de microarray. O protocolo demonstra passos desde a preparação da amostra até a análise de dados. Todos os procedimentos são descritos em detalhes, e o vídeo mostra os passos significativos.

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Genética Edição 183 metilação de DNA obesidade ChAMP bioinformática epigenética massa gorda índice de massa corporal
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