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Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium
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Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Article DOI: 10.3791/62619-v • 12:32 min • September 7th, 2021
September 7th, 2021 •
*1,2,3,4, *5, 1,2,3,4, 1,2,3,4, 5, 5, 1,2,3,4, 5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
* These authors contributed equally

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Summary
Automatic Translation

The present study reports a protocol for chromosome screening of human embryos that uses spent culture medium, which avoids embryo biopsy and enables chromosome ploidy identification using NGS. The present article presents the detailed procedure, including the preparation of culture medium, whole genome amplification (WGA), next-generation sequencing (NGS) library preparation, and data analysis.

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Chromosome Screening Human Preimplantation Embryos Spent Culture Medium Sample Collection Chromosomal Ploidy Analysis In Vitro Fertilization (IVF) Preimplantation Genetic Testing For Aneuploidy (PGT-A) Trophectoderm (TE) Inner Cell Mass (ICM) False Positives And Negatives NICS Technology DNA Sequencing Culture Medium Sampling Whole Genome Amplification (WGA) Library Preparation NGS Data Analysis Cryopreservation IVF Laboratory
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