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Journal / Genetics / Kromosomscreening af humane…
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JoVE Journal Genetics
Kromosomscreening af humane præimplantationsembryoner ved hjælp af brugt dyrkningsmedium
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Kromosomscreening af humane præimplantationsembryoner ved hjælp af brugt dyrkningsmedium: prøveindsamling og kromosomal ploidianalyse

Article DOI: 10.3791/62619-v • 12:32 min • September 7th, 2021
September 7th, 2021 •
*1,2,3,4, *5, 1,2,3,4, 1,2,3,4, 5, 5, 1,2,3,4, 5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
* These authors contributed equally

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Denne undersøgelse rapporterer en protokol til kromosomscreening af menneskelige embryoner, der bruger brugt dyrkningsmedium, som undgår embryobiopsi og muliggør kromosomploidiidentifikation ved hjælp af NGS. Denne artikel præsenterer den detaljerede procedure, herunder forberedelse af dyrkningsmedium, helgenomamplifikation (WGA), næste generations sekventering (NGS) biblioteksforberedelse og dataanalyse.

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Kromosomscreening Human præimplantationsembryoner brugt dyrkningsmedium prøveindsamling kromosomal ploidianalyse in vitro-befrugtning (IVF) præimplantationsgenetisk test for aneuploidier (PGT-A) trophectoderm (TE) indre cellemasse (ICM) falske positive og negative NICS-teknologi DNA-sekventering prøveudtagning af kulturmedium helgenomamplifikation (WGA) biblioteksforberedelse NGS-dataanalyse kryopræservering IVF-laboratorium
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