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JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

콜라겐 기반 스캐폴드를 사용한 신경모세포종의 3차원 체외 생체모방 모델

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Journal (Cancer Research)

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Journal (Cancer Research)

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

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콜라겐 기반 스캐폴드를 사용한 신경모세포종의 3차원 체외 생체모방 모델

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021

July 9th, 2021 •

Ciara Gallagher*^1,2,3, Catherine Murphy*^1,2,3, Graeme Kelly⁴, Fergal J. O’Brien^3,5,6, Olga Piskareva^1,2,3,6,7

¹Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ²School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ³Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ⁷Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, ⁵Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, ⁶Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, ⁷National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland

* These authors contributed equally

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Click here for the English version.

이 논문은 이전에 설명한 3차원 콜라겐 기반 스캐폴드에 신경모세포종 세포주를 파종하고, 미리 결정된 기간 동안 세포 성장을 유지하고, 다양한 실험 목표를 충족하도록 적응할 수 있는 여러 세포 성장 및 세포 행동 분석 및 다운스트림 애플리케이션을 위한 스캐폴드를 검색하는 데 필요한 단계를 나열합니다.

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3차원 체외 생체모방 모델 신경모세포종 콜라겐 기반 스캐폴드 소아암 종양 조직 세포외 기질(ECM) 질병 진행 환자 예후 치료 반응 신경모세포종 세포주 나노하이드록시아파타이트(nHA) 글리코사미노글리칸(GAG) 골수 전이 부위 다공성 구조 세포 부착 증식 이동 세포 클러스터 치료학 생체 내 상황 스캐폴드 기반 배양 시스템 2차원(2D) 세포 배양 DNA 정량화 세포 생존력

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