Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.
Kobayashi, H., Gieniec, K. A., Ng, J. Q., Goyne, J., Lannagan, T. R. M., Thomas, E. M., Radford, G., Wang, T., Suzuki, N., Ichinose, M., Wright, J. A., Vrbanac, L., Burt, A. D., Takahashi, M., Enomoto, A., Worthley, D. L., Woods, S. L. Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma. J. Vis. Exp. (175), e62630, doi:10.3791/62630 (2021).