Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Hiroki Kobayashi; Krystyna A. Gieniec; Jia Q. Ng; Jarrad Goyne; Tamsin R. M. Lannagan; Elaine M. Thomas; Georgette Radford; Tongtong Wang; Nobumi Suzuki; Mari Ichinose; Josephine A. Wright; Laura Vrbanac; Alastair D. Burt; Masahide Takahashi; Atsushi Enomoto; Daniel L. Worthley; Susan L. Woods

doi:10.3791/62630

Journal

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Cancer Research

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Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

6,406 Views

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07:59 min

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September 03, 2021

DOI:

10.3791/62630-v

DOI:

10.3791/62630-v

•

07:59 min

September 03, 2021

•

6390 Views

Hiroki Kobayashi^1,2,3,4, Krystyna A. Gieniec^1,2, Jia Q. Ng^1,2, Jarrad Goyne^1,2, Tamsin R. M. Lannagan^1,2, Elaine M. Thomas^1,2, Georgette Radford^1,2, Tongtong Wang^1,2, Nobumi Suzuki^1,2,5, Mari Ichinose^1,2, Josephine A. Wright², Laura Vrbanac^1,2, Alastair D. Burt⁶, Masahide Takahashi^3,4,7, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods^1,2

¹Adelaide Medical School,University of Adelaide ²South Australian Health and Medical Research Institute (SAHMRI)³Department of Pathology,Nagoya University Graduate School of Medicine ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer,Nagoya University Graduate School of Medicine ⁵Department of Gastroenterology, Graduate School of Medicine,The University of Tokyo ⁶Translational and Clinical Research Institute,Newcastle University ⁷International Center for Cell and Gene Therapy,Fujita Health University

Summary

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.