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Journal / Immunology and Infection / Enkeltcellekarakterisering af calciumtilstrømning …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Enkeltcellekarakterisering af calciumtilstrømning og HIV-1-infektion ved hjælp af en multiparameter optofluidisk platform
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Journal (Immunology and Infection)
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
Journal (Immunology and Infection)
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
 
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Enkeltcellekarakterisering af calciumtilstrømning og HIV-1-infektion ved hjælp af en multiparameter optofluidisk platform

Article DOI: 10.3791/62632-v • 07:15 min • May 18th, 2021
May 18th, 2021 •
1, 2, 3,4, 2, 1
1Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 3Black Family Stem Cell Institute, Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 4Sema4, a Mount Sinai venture

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Her præsenterer vi en protokol, hvor enkelte celler overvåges for akutte hændelser og produktiv HIV-1-infektion på en nanofluidisk enhed. Billeddata definerer virusværtsreceptorinteraktioner og signalvejsdynamik. Dette er den første metode til nanofluidic high-throughput langsgående enkeltcellekultur og billeddannelse til at studere signalering af kinetik og molekylære interaktioner.

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Immunologi og infektion Problem 171 høj-hele optofluidic levende celle billeddannelse HIV-infektion calcium tilstrømning purinergisk receptor
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