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Journal / Immunology and Infection / Encellet karakterisering av kalsiumtilstrømning…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Encellet karakterisering av kalsiumtilstrømning og HIV-1-infeksjon ved hjelp av en multiparameter optofluidisk plattform
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Encellet karakterisering av kalsiumtilstrømning og HIV-1-infeksjon ved hjelp av en multiparameter optofluidisk plattform

Article DOI: 10.3791/62632-v • 07:15 min • May 18th, 2021
May 18th, 2021 •
1, 2, 3,4, 2, 1
1Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 3Black Family Stem Cell Institute, Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 4Sema4, a Mount Sinai venture

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Her presenterer vi en protokoll der enkeltceller overvåkes for akutte hendelser og produktiv HIV-1-infeksjon på en nanofluidisk enhet. Bildedata definerer interaksjoner mellom virusvertsreseptorer og signalveidynamikk. Dette er den første metoden for nanofluidisk høygjennomstrømning langsgående encellet kultur og avbildning for å studere signalkinetikk og molekylære interaksjoner.

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Immunologi og infeksjon utgave 171 gjennomgående optofluidisk levende celleavbildning HIV-infeksjon kalsiumtilstrømning purinergisk reseptor
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