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Journal / Biology / 5-Ethynyl-2'-Desoxyuridin/Phospho-Histon H3…
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5-Ethynyl-2'-Desoxyuridin/Phospho-Histon H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells

Article DOI: 10.3791/62642-v • 09:34 min • May 4th, 2021
May 4th, 2021 •
1,2, 1
1Cell Biology, University of Bern, 2Department of life sciences and medicine, University of Luxembourg

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Die Zellzyklusanalyse mit 5-Ethynyl-2'-desoxyuridin (EdU) und Phospho-Histon H3 (pH3) Markierung ist ein mehrstufiges Verfahren, das eine umfangreiche Optimierung erfordern kann. Hier stellen wir ein detailliertes Protokoll vor, das alle Schritte für dieses Verfahren einschließlich Bildanalyse und Quantifizierung beschreibt, um Zellen in verschiedenen Zellzyklusphasen zu unterscheiden.

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Biologie Heft 171
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