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Immunology and Infection

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JoVE Journal Immunology and Infection
Makrofag differensiering og polarisering i en M2-lignende fenotype ved hjelp av en human monocyttlignende THP-1 leukemicellelinje
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Makrofag differensiering og polarisering i en M2-lignende fenotype ved hjelp av en human monocyttlignende THP-1 leukemicellelinje

Article DOI: 10.3791/62652-v • 06:38 min • August 2nd, 2021
August 2nd, 2021 •
1, 1, 1, 1, 1
1Price Institute of Surgical Research, Department of Surgery, University of Louisville

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M2-lignende tumorrelaterte makrofager (TAM) er forbundet med tumorprogresjon og dårlig prognose hos kreft. Denne protokollen fungerer som en detaljert veiledning for å reprodusere og polarisere THP-1 monocyttlignende celler i M2-lignende makrofager innen 14 dager. Denne modellen er grunnlaget for å undersøke de antiinflammatoriske effektene av TAM i tumormikromiljøet.

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Immunologi og infeksjon utgave 174
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