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Journal / Developmental Biology / 2D en 3D humane geïnduceerde pluripotente…
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Developmental Biology
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JoVE Journal Developmental Biology
2D en 3D humane geïnduceerde pluripotente stamcelgebaseerde modellen om primaire ciliumbetrokkenheid tijdens neocorticale ontwikkeling te ontleden
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2D en 3D humane geïnduceerde pluripotente stamcelgebaseerde modellen om primaire ciliumbetrokkenheid tijdens neocorticale ontwikkeling te ontleden

Article DOI: 10.3791/62667-v • 14:19 min • March 25th, 2022
March 25th, 2022 •
*1, *1, 2, 1, 1, 1, 3, 1, 1, 1, 4, 1, 1, 1,5, 1,6, 2, 1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
* These authors contributed equally

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We presenteren gedetailleerde protocollen voor de generatie en karakterisering van 2D en 3D human induced pluripotent stamcel (hIPSC)-gebaseerde modellen van neocorticale ontwikkeling, evenals complementaire methodologieën die kwalitatieve en kwantitatieve analyse van primaire cilium (PC) biogenese en functie mogelijk maken.

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Ontwikkelingsbiologie Nummer 181 primair cilium door de mens geïnduceerde pluripotente stamcellen hIPSCs neurale stam- en voorlopercellen menselijke cerebrale corticale ontwikkeling neurale rozetten dorsale voorhersenenorganoïden 2D- en 3D hIPSC-gebaseerde modellen van neocorticale ontwikkeling cerebrale organoïden Sonic hedgehog whole-mount immunostaining optische clearing lichtbladmicroscoop
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