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JoVE Journal Developmental Biology

2Dおよび3Dヒト人工多能性幹細胞ベースのモデルにより、新皮質発生中の原発性繊毛の関与を解剖

Imaging Calcium in Drosophila at Egg Activation

Journal (Developmental Biology)

Imaging Calcium in Drosophila at Egg Activation

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Journal (Developmental Biology)

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

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2Dおよび3Dヒト人工多能性幹細胞ベースのモデルにより、新皮質発生中の原発性繊毛の関与を解剖

Article DOI: 10.3791/62667-v • 14:19 min • March 25th, 2022

March 25th, 2022 •

Lucile Boutaud*¹, Marie Michael*¹, Céline Banal², Damelys Calderon¹, Sarah Farcy¹, Julie Pernelle¹, Nicolas Goudin³, Camille Maillard¹, Clémantine Dimartino¹, Cécile Deleschaux¹, Sébastien Dupichaud⁴, Corinne Lebreton¹, Sophie Saunier¹, Tania Attié-Bitach^1,5, Nadia Bahi-Buisson^1,6, Nathalie Lefort², Sophie Thomas¹

¹Imagine Institute, INSERM UMR 1163, Université de Paris, ²Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, ³Necker Bio-image Analysis platform of the SFR Necker, ⁴Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, ⁵Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, ⁶Pediatric Neurology, APHP- Necker Enfants Malades Hospital

* These authors contributed equally

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我々は、新皮質発生の2Dおよび3Dヒト人工多能性幹細胞(hIPSC)ベースのモデルの生成および特性評価のための詳細なプロトコル、ならびに初代繊毛(PC)生合成および機能の定性的および定量的分析を可能にする補完的方法論を提示する。

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発生生物学初代繊毛ヒト人工多能性幹細胞 hIPSC 神経幹および前駆細胞ヒト脳皮質発達神経ロゼット背側前脳オルガノイド 2Dおよび3D hIPSCベースの新皮質発生モデル脳オルガノイドソニックヘッジホッグホールマウント免疫染色光クリアリングライトシート顕微鏡

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