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Journal / Immunology and Infection / Isolering av livmor medfødte lymfoide celler for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av livmor medfødte lymfoide celler for analyse ved flowcytometri
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Isolering av livmor medfødte lymfoide celler for analyse ved flowcytometri

Article DOI: 10.3791/62670-v • 09:02 min • October 14th, 2021
October 14th, 2021 •
*1,2, *1,2, *1,2, 1,2, 1, 1,2
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research, University of Cambridge
* These authors contributed equally

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Dette er en metode for å isolere livmor lymfoide celler fra både gravide og ikke-gravide mus. Denne metoden kan brukes til flere nedstrømsprogrammer som FACS-fenotyping, cellesortering, funksjonelle analyser, RNA-seq og proteomikk. Protokollen her demonstrerer hvordan fenotype gruppe 1 livmor medfødte lymfoide celler ved strømning cytometri.

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Immunologi og infeksjon Utgave 176 graviditet livmor decidua naturlige morderceller medfødte lymfoidceller mus
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