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Chemistry
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基于毛细管电泳的氢/氘交换,用于自上而下的质谱法对蛋白质进行构象表征
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Article
DOI:
10.3791/62672-v
•
05:45 min
•
June 8th, 2021
基于毛细管电泳的氢/氘交换,用于自上而下的质谱法对蛋白质进行构象表征
June 8th, 2021
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
这里介绍的是基于毛细管电泳的氢/氘交换(HDX)方法与自上而下的质谱法相结合的方案。该方法通过同时进行差分HDX和电泳分离来表征不同蛋白质物种(包括不同状态的蛋白质和不同蛋白质形式)之间高阶结构的差异。