Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Chemistry

A subscription to JoVE is required to view this content.

基于毛细管电泳的氢/氘交换,用于自上而下的质谱法对蛋白质进行构象表征
 
Click here for the English version

基于毛细管电泳的氢/氘交换,用于自上而下的质谱法对蛋白质进行构象表征

Article DOI: 10.3791/62672-v 05:45 min June 8th, 2021
June 8th, 2021

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

这里介绍的是基于毛细管电泳的氢/氘交换(HDX)方法与自上而下的质谱法相结合的方案。该方法通过同时进行差分HDX和电泳分离来表征不同蛋白质物种(包括不同状态的蛋白质和不同蛋白质形式)之间高阶结构的差异。

Tags

化学, 第172期,
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter