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Journal / Developmental Biology / Nuclear Migration in the Drosophila Oocyte
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JoVE Journal Developmental Biology
Nuclear Migration in the Drosophila Oocyte
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Nuclear Migration in the Drosophila Oocyte

Article DOI: 10.3791/62688-v • 04:17 min • May 13th, 2021
May 13th, 2021 •
1, 1, 1
1Université de Paris, CNRS, Institut Jacques Monod

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In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.

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Nuclear Migration Drosophila Oocyte Live Imaging Microscopy Dissected Egg Chambers Schneider Medium Insulin Supplement Fetal Calf Serum Silicone Grease Coverslip Dissecting Well Imaging Medium Forceps Uterus Oviduct Muscle Sheath Ovaries Ovarioles Germarium
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