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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Article DOI: 10.3791/62701-v • 04:37 min • June 29th, 2021
June 29th, 2021 •
1, 2, 2
1Department of Chemistry, Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University

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We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.

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High-pressure NMR Experiments Protein Conformational States Pressure Perturbation Nitrogen-15-labeled Sample Zirconia Tube Glass Pipette Mineral Oil Overlay Transmission Liquid High-pressure Tether Line Torque Pressure Cell Assembly Spectrometer
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