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Coleta de tecidos e extração de RNA da articulação do joelho osteoartrítica humana
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Coleta de tecidos e extração de RNA da articulação do joelho osteoartrítica humana

Article DOI: 10.3791/62718-v • 06:06 min • July 22nd, 2021
July 22nd, 2021 •
1, 1, 2, 1,3
1Bone and Joint Center, Henry Ford Health System, 2Department of Orthopedic Surgery, Henry Ford Health System, 3Center for Molecular Medicine and Genetics, Wayne State University

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Os tecidos primários obtidos de pacientes após a artroplastia total do joelho fornecem um modelo experimental para a pesquisa da osteoartrite com tradução clínica máxima. Este protocolo descreve como identificar, processar e isolar o RNA de sete tecidos únicos do joelho para apoiar a investigação mecanicista na osteoartrite humana.

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Biologia Edição 173
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