Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / 在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
Journal (Immunology and Infection)
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
Selective Harvesting of Marginating-hepatic Leukocytes
Journal (Immunology and Infection)
Selective Harvesting of Marginating-hepatic Leukocytes
 
Click here for the English version

在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究

Article DOI: 10.3791/62727-v • 07:21 min • June 30th, 2021
June 30th, 2021 •
1, *1, *1, *1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

在这里，我们提供了使用在细胞堆叠中生长的贴壁HEK 293细胞和亲和色谱纯化大规模生产研究级AAV载体的详细程序。该方案始终如一地产生>1 x 1013 个载体基因组/ mL，提供适合大型动物研究的载体量。

Tags

免疫学和感染，第172期，腺相关病毒载体，基因治疗，细胞堆栈，亲和纯化，临床前研究，大型动物模型，转基因表达，AAV6，AAV6.2FF
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter