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JoVE Journal
Bioengineering

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使用无细胞系统对用于蛋白质原型的最小线性模板进行扩增的快速酶促方法
 
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使用无细胞系统对用于蛋白质原型的最小线性模板进行扩增的快速酶促方法

Article DOI: 10.3791/62728-v
June 14th, 2021

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Summary June 14th, 2021

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该研究描述了一种方案,用于在不克隆或使用活细胞的情况下从合成基因片段中创建大量(μg-mg)DNA以进行蛋白质筛选活动。将最小模板酶消化并循环化,然后使用等温滚动循环扩增进行扩增。无细胞表达反应可以用未纯化的产物进行。

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