Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Medición del pH, las químicas redox y la…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Medición del pH, las químicas redox y la capacidad degradativa de los macropinosomas mediante microscopía ratiométrica de fluoróforo dual
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
Journal (Immunology and Infection)
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Journal (Immunology and Infection)
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
 
Click here for the English version

Medición del pH, las químicas redox y la capacidad degradativa de los macropinosomas mediante microscopía ratiométrica de fluoróforo dual

Article DOI: 10.3791/62733-v • 07:31 min • August 19th, 2021
August 19th, 2021 •
1,2, 2,3
1Department of Biochemistry & Microbiology, Faculty of Science, University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science, University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, University of Calgary

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Describimos protocolos para medir el pH, los eventos oxidativos y la digestión de proteínas en macropinosomas individuales en células vivas. Se hace hincapié en la microscopía ratiométrica de fluoróforos duales y las ventajas que ofrece sobre las técnicas basadas en la población.

Tags

Inmunología e Infección Número 174 macropinocitosis macropinosoma endocitosis pH V-ATPasa bomba de protones especies reactivas de oxígeno ROS NADPH oxidasa fagocito macrófago célula dendrítica
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter