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Produzione rapida, economica e semplice di lisato privo di cellule batteriche
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Produzione rapida, economica e semplice di lisato privo di cellule batteriche

Article DOI: 10.3791/62753-v • 06:03 min • October 29th, 2021
October 29th, 2021 •
*1, *1,2, *1,3, 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
* These authors contributed equally

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Questo protocollo descrive un metodo rapido e semplice per produrre lisato batterico per l'espressione genica senza cellule, utilizzando un ceppo ingegnerizzato di Escherichia coli e richiedendo solo attrezzature di laboratorio standard.

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Biologia Numero 176
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