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Snabb, prisvärd och okomplicerad produktion av bakteriell cellfri lysate
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Snabb, prisvärd och okomplicerad produktion av bakteriell cellfri lysate

Article DOI: 10.3791/62753-v • 06:03 min • October 29th, 2021
October 29th, 2021 •
*1, *1,2, *1,3, 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
* These authors contributed equally

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Detta protokoll beskriver en snabb och enkel metod för att producera bakteriell lysate för cellfria genuttryck, med hjälp av en konstruerad stam av Escherichia coli och kräver endast standard laboratorieutrustning.

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Biologi nummer 176
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