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Journal / Biochemistry / Estudios de interacciones chaperona-cochaperona…
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Biochemistry

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JoVE Journal Biochemistry
Estudios de interacciones chaperona-cochaperona utilizando un ensayo homogéneo basado en cuentas
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Estudios de interacciones chaperona-cochaperona utilizando un ensayo homogéneo basado en cuentas

Article DOI: 10.3791/62762-v • 06:51 min • July 21st, 2021
July 21st, 2021 •
1, 1, 1,2, 1,3, 1
1Department of Neuroscience Care and Society, Division of Neurogeriatrics, Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), 3Theme Inflammation and Aging, Karolinska University Hospital

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Este protocolo presenta una técnica para sondear las interacciones proteína-proteína utilizando perlas de donantes ligadas al glutatión con co-chaperonas con motivos TPR fusionadas con GST y perlas aceptoras junto con un péptido derivado de Hsp90. Hemos utilizado esta técnica para detectar moléculas pequeñas para interrumpir las interacciones Hsp90-FKBP51 o Hsp90-FKBP52 e identificado inhibidores potentes y selectivos de la interacción Hsp90-FKBP51.

Tags

Bioquímica Número 173 AlphaScreen interacciones proteína-proteína chaperona molecular Hsp90 FKBP51 FKBP52 proteínas TPR cribado de compuestos
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