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Developmental Biology

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JoVE Journal Developmental Biology
Analyse av transformerende vekstfaktor ß Family Cleavage Products utskilt til Blastocoele av Xenopus laevis Embryos
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Analyse av transformerende vekstfaktor ß Family Cleavage Products utskilt til Blastocoele av Xenopus laevis Embryos

Article DOI: 10.3791/62782-v • 06:57 min • July 21st, 2021
July 21st, 2021 •
1, 2
1Department of Neurobiology, University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine

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Når Transforming Growth Factor ß familie forløper proteiner er ektopisk uttrykt i Xenopus laevis embryoer, de dimerize, bli cleaved og utskilles i blastocoele, som begynner på sen blastula til tidlig gastrula stadium. Vi beskriver en metode for aspirerende spaltingsprodukter fra blastocoele-hulrommet for immunoblotanalyse.

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Utviklingsbiologi utgave 173
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