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Bestämning av trepartsinteraktion mellan tvåonomerer av en MADS-box transkriptionsfaktor och ett kalciumsensorprotein av BiFC-FRET-FLIM-analys…
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Bestämning av trepartsinteraktion mellan två monomerer av en MADS-box transkriptionsfaktor och ett kalciumsensorprotein av BiFC-FRET-FLIM-analys
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Article
DOI:
10.3791/62791-v
•
14:34 min
•
December 25th, 2021
Bestämning av trepartsinteraktion mellan två monomerer av en MADS-box transkriptionsfaktor och ett kalciumsensorprotein av BiFC-FRET-FLIM-analys
December 25th, 2021
•
Neelima Boora*1, Vibha Verma*1, Ridhi Khurana1, Gautam Gawande1, Sanchi Bhimrajka1, Komal Chaprana1, Meenu Kapoor2, Sanjay Kapoor1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Här presenterar vi, en metod för att visualisera ternary komplex bildning mellan tre proteinpartners med fluorescerande-märkta proteiner av BiFC-baserade FRET-FLIM analys. Denna metod är värdefull för att studera protein-protein interaktionskomplex in vivo.