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Biology
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通过BiFC-FRET-FLIM测定MADS盒转录因子和钙传感器蛋白两种单体之间的三方相互作用
 
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通过BiFC-FRET-FLIM测定MADS盒转录因子和钙传感器蛋白两种单体之间的三方相互作用

Article DOI: 10.3791/62791-v 14:34 min December 25th, 2021
December 25th, 2021

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在这里,我们提出了一种通过基于BiFC的FRET-FLIM测定使用荧光标记蛋白可视化三元复合物形成的方法。该方法对于研究 体内蛋白质 - 蛋白质相互作用复合物很有价值。

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生物学,第178期,
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