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Bestemmelse af trepartsinteraktion mellem to monomerer af en MADS-box transskriptionsfaktor og et calciumsensorprotein ved BiFC-FRET-FLIM Assay
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Bestemmelse af trepartsinteraktion mellem to monomerer af en MADS-box transskriptionsfaktor og et calciumsensorprotein ved BiFC-FRET-FLIM Assay

Article DOI: 10.3791/62791-v • 14:34 min • December 25th, 2021
December 25th, 2021 •
*1, *1, 1, 1, 1, 1, 2, 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally

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Her præsenterer vi, en metode til at visualisere ternary kompleks dannelse mellem tre proteinpartnere ved hjælp af fluorescerende-tagged proteiner ved BiFC baseret FRET-FLIM assay. Denne metode er værdifuld til undersøgelse af protein-protein interaktion komplekser in vivo.

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Biologi udgave 178
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