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Journal / Developmental Biology / Generation av naiva Blastoderm Explants från…
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Developmental Biology

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JoVE Journal Developmental Biology
Generation av naiva Blastoderm Explants från Zebrafish Embryon
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Generation av naiva Blastoderm Explants från Zebrafish Embryon

Article DOI: 10.3791/62797-v • 07:21 min • July 30th, 2021
July 30th, 2021 •
1, 1
1Center for Precision Environmental Health and Department of Molecular and Cellular Biology, Baylor College of Medicine

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Zebrafish blastoderm explants genereras genom att isolera embryonala celler från endogena signalcentra inom det tidiga embryot, vilket producerar relativt naiva cellkluster som lätt manipuleras och odlas ex vivo. Den här artikeln innehåller instruktioner för att göra sådana explanter och demonstrerar deras användbarhet genom att förhöra roller för Nodal-signalering under gastrulation.

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Utvecklingsbiologi nummer 173
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