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Bioengineering

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JoVE Journal Bioengineering
Automatisert mikrobiell dyrking og adaptiv evolusjon ved hjelp av mikrobielt mikrodropletkultursystem (MMC)
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Automatisert mikrobiell dyrking og adaptiv evolusjon ved hjelp av mikrobielt mikrodropletkultursystem (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

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Denne protokollen beskriver hvordan du bruker det mikrobielle mikrodropletkultursystemet (MMC) til å utføre automatisert mikrobiell dyrking og adaptiv evolusjon. MMC kan dyrke og underdyrke mikroorganismer automatisk og kontinuerlig og overvåke veksten på nettet med relativt høy gjennomstrømning og god parallellisering, noe som reduserer arbeids- og reagensforbruket.

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Bioengineering Utgave 180 mikrobielt mikrodropletkultursystem automatiserte operasjoner høy gjennomstrømning mikrobiell dyrking adaptiv laboratorieutvikling online deteksjon
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