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Journal / Bioengineering / Cultivo Microbiano Automatizado e Evolução…
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Bioengineering

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JoVE Journal Bioengineering
Cultivo Microbiano Automatizado e Evolução Adaptativa usando Sistema de Cultura microbiana de microdroplet (MMC)
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Cultivo Microbiano Automatizado e Evolução Adaptativa usando Sistema de Cultura microbiana de microdroplet (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

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Este protocolo descreve como usar o sistema microbiano de cultura de microdroplet (MMC) para realizar o cultivo microbiano automatizado e a evolução adaptativa. A MMC pode cultivar e subsu cultivar microrganismos automaticamente e continuamente e monitorar on-line seu crescimento com rendimento relativamente alto e boa paraleloização, reduzindo o consumo de mão-de-obra e reagente.

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Bioengenharia Edição 180 sistema de cultura microbiana de microdroplet operações automatizadas alto rendimento cultivo microbiano evolução adaptável do laboratório detecção on-line
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