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Imagerie des domaines réplicatifs dans la chromatine ultrastructurellement préservée par tomographie électronique
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Imagerie des domaines réplicatifs dans la chromatine ultrastructurellement préservée par tomographie électronique

Article DOI: 10.3791/62803-v • 14:56 min • May 20th, 2022
May 20th, 2022 •
*1,2, *1,2, *1,3, 1,2, 1, 1, 1, 2, 4, 1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
* These authors contributed equally

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Ce protocole présente une technique de cartographie à haute résolution des sites de réplication dans la chromatine structurellement préservée in situ qui utilise une combinaison de marquage pré-intégration EdU-streptavidin-Nanogold et ChromEMT.

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Biologie numéro 183
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