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Domini replicativi di imaging in cromatina conservata ultrastrutturalmente mediante tomografia elettronica…
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Domini replicativi di imaging in cromatina conservata ultrastrutturalmente mediante tomografia elettronica
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Article
DOI:
10.3791/62803-v
•
14:56 min
•
May 20th, 2022
Domini replicativi di imaging in cromatina conservata ultrastrutturalmente mediante tomografia elettronica
May 20th, 2022
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Sophie Sosnovskaya*1,2, Amir N. Zakirov*1,2, Ekaterina D. Ryumina*1,3, Ekaterina Kharybina1,2, Sergei A. Golyshev1, Olga S. Strelkova1, Oxana A. Zhironkina1, Andrei Moiseenko2, Anton Orekhov4, Igor I. Kireev1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Questo protocollo presenta una tecnica per la mappatura ad alta risoluzione dei siti di replicazione in cromatina strutturalmente conservata in situ che impiega una combinazione di marcatura EdU-streptavidin-Nanogold pre-incorporante e ChromEMT.