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JoVE Journal
Cancer Research

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In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
 

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Article DOI: 10.3791/62811-v 08:02 min September 23rd, 2021
September 23rd, 2021

Chapters

Summary

This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings.

Tags

In Vivo Immunogenicity Screening Tumor-derived Extracellular Vesicles Flow Cytometry Splenic T Cells Cancer Immunotherapy Personalized Anti-cancer Treatment Autologous Extracellular Vesicles Cell Culture Media Ultracentrifugation B16 Melanoma Cells Ovalbumin Expression Oxaliplatin Treatment
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