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Bioengineering
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构建含有G蛋白偶联受体(GPCR)的脂质膜模型
 
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构建含有G蛋白偶联受体(GPCR)的脂质膜模型

Article DOI: 10.3791/62830-v 09:45 min February 5th, 2022
February 5th, 2022

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该协议利用琼脂糖肿胀作为一种强大且可推广的技术,用于将整体膜蛋白(IMP)掺入巨型单层脂质囊泡(GUV)中,如本文所述,用于重建人1A 5-羟色胺受体蛋白(5-HT1AR),这是药理学上重要的G蛋白偶联受体之一。

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生物工程,第180期,
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