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Journal / Immunology and Infection / Brugervenlig, høj-throughput, og fuldautomatisk…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Brugervenlig, høj-throughput, og fuldautomatisk dataindsamling software til single-partikel Cryo-elektron Mikroskopi
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Brugervenlig, høj-throughput, og fuldautomatisk dataindsamling software til single-partikel Cryo-elektron Mikroskopi

Article DOI: 10.3791/62832-v • 07:56 min • July 29th, 2021
July 29th, 2021 •
1, 1, 2, 1
1Molecular Biophysics Unit, Indian Institute of Science, 2Gatan Inc.

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Enkelt-partikel cryo-elektronmikroskopi kræver en passende softwarepakke og brugervenlig rørledning til automatisk dataindsamling med høj kapacitet. Her præsenterer vi anvendelsen af en fuldautomatisk softwarepakke til billedopkøb, Latitude-S, og en praktisk pipeline til dataindsamling af vitrified biomolekyler under lavdosisforhold.

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Immunologi og infektion Udgave 173 cryo-EM Latitude-S Enkelt partikelanalyse SARS-CoV-2 Spike-Protein Talos Arctica
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