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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

Article DOI: 10.3791/62834-v • 06:34 min • May 31st, 2022
May 31st, 2022 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, 2Wellcome Centre for Human Genetics, University of Oxford
* These authors contributed equally

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Summary
Automatic Translation

This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.

Tags

Isolation In Vitro Culture Bone Marrow-derived Macrophages INOS Deficient Mice BH4 Deficient Mice Defined Media Low Levels Of BH4 Nitrate Interference Redox Mechanisms Potential Contaminants Accuracy Reproducibility Biopterins Nitric Oxide Downstream Factors Measurements Dr. Thomas Nichol Post-doctoral Researcher Legs 70% Ethanol Fur Residue Bacteriological Petri Dish Scrape Bones Tendons Muscle Removal Tibia Femur Knee Joint Hip Joint Bone Marrow Flushing PBS
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