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Journal / Immunology and Infection / Isolatie en in vitro kweek van beenmerg-afgeleide …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Isolatie en in vitro kweek van beenmerg-afgeleide macrofagen voor de studie van NO-redox biologie
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Isolatie en in vitro kweek van beenmerg-afgeleide macrofagen voor de studie van NO-redox biologie

Article DOI: 10.3791/62834-v • 06:34 min • May 31st, 2022
May 31st, 2022 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, 2Wellcome Centre for Human Genetics, University of Oxford
* These authors contributed equally

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Dit protocol is vastgesteld voor het kweken van tetrahydrobiopterine (BH4) - en induceerbare stikstofmonoxidesynthase (iNOS) - deficiënte primaire murine macrofagen om NO-redox biologie te bestuderen. De studie richt zich op het verminderen van potentiële besmetting van BH4 en andere artefacten gevonden in traditionele isolatie- en kweekmethoden die experimentele uitkomsten en interpretatie van resultaten kunnen verstoren.

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Immunologie en infectie nummer 183
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