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Ekstracellulær vesicle opptak analyse via confocal mikroskop imaging analyse
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Ekstracellulær vesicle opptak analyse via confocal mikroskop imaging analyse

Article DOI: 10.3791/62836-v • 08:32 min • February 14th, 2022
February 14th, 2022 •
*1,2, *1, 1,3, 2,4, 1, 2,4, 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)
* These authors contributed equally

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Ekstracellulære vesicles (ELBILER) bidrar til cellulær biologi og intercellulær kommunikasjon. Det er behov for praktiske analyser for å visualisere og kvantifisere elbiler opptak av cellene. Den nåværende protokollen foreslår EV-opptaksanalysen ved å bruke tredimensjonal fluorescensavbildning via konfokal mikroskopi, etter EV-isolasjon av en nanofiltreringsbasert mikrofluidisk enhet.

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Biologi utgave 180
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