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Aislamiento de neutrófilos humanos de sangre entera y buffy coats
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Aislamiento de neutrófilos humanos de sangre entera y buffy coats

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

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Este protocolo detalla un método para el aislamiento de neutrófilos de sangre total, capas buffy o membranas de leucoféresis, logrando un buen rendimiento, alta pureza y mínima activación celular. Utilizamos la purificación por gradiente, la sedimentación de glóbulos rojos (RBC) y la lisis de RBC para obtener una preparación de neutrófilos de alta calidad / pureza.

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Biología Número 175 Neutrófilo gradiente dextrano lisis de glóbulos rojos sangre total pelaje buffy
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