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Isolement des neutrophiles humains du sang total et des buffys

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

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Ce protocole détaille une méthode pour isoler les neutrophiles du sang total, des manteaux bouffants ou des membranes de leucaphérèse, obtenant un bon rendement, une pureté élevée et une activation cellulaire minimale. Nous utilisons la purification par gradient, la sédimentation des globules rouges (RBC) et la lyse des globules rouges pour obtenir une préparation de neutrophiles de haute qualité / pureté.

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Biologie numéro 175 Neutrophile gradient dextran lyse des globules rouges sang total buffy coat
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