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Journal / Immunology and Infection / Un sistema di coltura automatizzato da utilizzare …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un sistema di coltura automatizzato da utilizzare nei test preclinici di terapie dirette all'ospite per la tubercolosi
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Un sistema di coltura automatizzato da utilizzare nei test preclinici di terapie dirette all'ospite per la tubercolosi

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021
August 16th, 2021 •
1, 1,2, 2, 2,3,4, 1, 1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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La quantificazione rapida ed efficiente della crescita intracellulare di M. tuberculosis è fondamentale per perseguire terapie migliori contro la tubercolosi (TB). Questo protocollo descrive un test di rilevamento colorimetrico basato sul brodo utilizzando un sistema di coltura liquida automatizzato per quantificare la crescita di Mtb nei macrofagi trattati con terapie candidate dirette dall'ospite.

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Immunologia e infezione numero 174
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